Characterization of aromatic hydrocarbon rading bacteria from. Medina hydrocarbon degrading bacteria consists of known nonpathogenic microbial strains that degrade petroleum hydrocarbons in solid and aqueous environments. Although oil pollution is difficult to treat, petroleum hydrocarbondegrading bacteria have evolved as a result of existing in close proximity to. Study of oil degrading bacteria isolated from oil contaminated sites. Pdf hydrocarbon degrading bacteria as indicator of petroleum. Supplied in a dry bran mixture, microorganisms are activated when mixed with water. The prepared amplicons are transformed into non hydrocarbon degrading bacteria. Characterization of aromatic hydrocarbon degrading bacteria from petroleum contaminated sites 245. Characterization of aromatic hydrocarbon rading bacteria. Anaerobic degradation of oil hydrocarbons by sulfatereducing. Consistent oil spill gives the soil a greasy feel which cake at the soil surface. Realtime pcr is now widely employed for measuring 16s rrna and functional gene abundance and expression in the environment, has been used in numerous studies of hydrocarbondegrading bacteria, and the technique has promising possibilities as a tool for assessing hydrocarboncontaminated environments and monitoring natural attenuation or.
Presence of opportunistic oildegrading microorganisms operating. Free fulltext pdf articles from hundreds of disciplines, all in one place polycyclic aromatic hydrocarbondegrading bacteria from aviation fuel spill site at ibeno, nigeria pdf paperity toggle navigation. The prepared amplicons are transformed into nonhydrocarbon degrading bacteria. The level of petroleum hydrocarbon degradation was determined by gravimetric assay at each 5 days interval. Department of chemistry, rochester institute of technology, rochester, ny. These compounds can be transformed by hydrocarbon degrading microorganisms which occur naturally in the environment 3,4. Accidental releases of petroleum products are of particular concern in the environment. Pdf anaerobic hydrocarbondegrading deltaproteobacteria. Researches made over the last decade have led to the emergence of new and advanced technologies that use biological agents to remediate contaminated soils 10. In the present study, altogether 32 morphologically distinct bacteria were isolated from crude oil contaminated soil and 18 different hydrocarbon degrading bacteria were number hydrocarbon degrading bacterial strain from and.
Isolation of hydrocarbon degrading bacteria following the strategy designed in our work to isolate hydrocarbondegrading bacteria both with high growth and biological activity, 39 purified bacterial isolates were recorded amongst hundreds of isolated ones at the first step of the program. Bayoumi and abulhamd 2010, have reported isolation of one hundred and nine 109 pure bacterial cultures in mineral salt medium msm supplemented with toluene and phenol. Hydrocarbondegrading bacteria and the bacterial community response in gulf of mexico beach sands impacted by the deepwater horizon oil spill joel e. Sep 14, 2016 created using powtoon free sign up at create animated videos and animated presentations for free. Frontiers petroleum hydrocarbondegrading bacteria for. In previous studies, two hydrocarbondegrading sulfatereducing bacteria, strains pp31t and pl12t, were obtained from oilpolluted marine sediments of shuaiba, kuwait.
Most importantly, hydrocarbonoclastic bacteria will decrease the concentration and toxicity of hydrocarbons in the immediate vicinity of algal cells. They had been reported as organisms capable of anaerobic degradation of pxylene and nalkanes, respectively. Isolation and characterization of hydrocarbondegrading bacteria. Indeed, the proportion of hydrocarbon degrading bacteria at 10,400 m is the highest observed in any natural environment on earth. Isolation and characterization of hydrocarbon degrading bacteria from environmental habitats in western new york state, masters thesis in chemistry, 2005. Hydrocarbondegrading bacteria and the bacterial community. Estimation of protein in hydrocarbon degrading bacteria. S1 supporting information succession of hydrocarbon degrading bacteria in the aftermath of the deepwater horizon oil spill in the gulf of mexico eric a. One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Raeid m m abed, jamal alsabahi, fatema almaqrashi, amal al.
That study assumed that the consumption of alkanes in the enrichments was performed by consortia of alkanedegrading bacteria and methanogenic archaea. Screening and isolation of a hydrocarbon degrading. Under aerobic conditions, aromatic hydrocarbons can be oxidized by bacteria via oxygenases, enzymes that catalyze the nadphdependent incorporation of molecular oxygen into the aromatic ring or alkyl substituent. In the same sand samples for which detailed chemical analysis was performed, mpn counts of cultivatable hydrocarbondegrading bacteria in oilcontaminated sands 2. The transformation was successful and the transformed bacteria showed good growth in bushnell haas media with diesel as the sole source of carbon. Based on this information, we experimentally tested and confirmed the hydrocarbon degradation activity of. Agar plates without heavy metals served as control. Amb express effect of high pressure on hydrocarbondegrading. Enrichment, isolation and identification of hydrocarbon degrading. May 17, 2017 hydrocarbonoclastic bacteria hcb play a key role in the biodegradation of oil hydrocarbons in marine and other environments.
Introduction hydrocarbon pollution is one of the important concerns in the developing countries. Supporting information succession of hydrocarbondegrading. Feb 06, 20 a total of 1 strains of hydrocarbon degrading bacteria were isolated from the surface sediment samples, during the period between july 2009 june 2010, slightly predominated by gramnegative bacteria. Anaerobic degradation of oil hydrocarbons by sulfate. Isolation and identification of hydrocarbon degrading. Green,1,3 gina freyer,1,2 andy canion, jonathan delgardio, 1nikita norton, terry c. Identification of hydrocarbondegrading bacteria in soil by reverse. However, cultures have not been preserved, or attempts to reproduce hydrocarbon oxidation by desulfovibrio strains failed 1, 2. Crude oil petroleum is a highly complex mixture of organic compounds of which some 1.
Isolation and characterization of hydrocarbondegrading. Hydrocarbon degrading bacteria could supply the algae with the benefits outlined in figure 4. Isolation and identification of petroleum hydrocarbon. Proliferation of hydrocarbondegrading microbes at the bottom. Petroleum hydrocarbondegrading bacteria and the type of petroleum components they degrade are listed in table 1. The detection limit of the pcr amplification was 10. An unexpected enrichment for hydrocarbon degrading bacteria, along with an increase in the abundance and transcription of hydrocarbon degrading genes, was found at depths near the trench bottom 10,400m and 10,500 m.
Source of isolation the source for bacterial isolation consisted of wastewaters contaminated with. Hydrocarbondegrading microorganisms from natural environments have been isolated and. Petroleum hydrocarbon degrading bacteria are ubiquitous in nature and can utilize these compounds as sources of carbon and energy. Zulfa aldisi1, samir jaoua1, dhabia althani1, saeed. With the sharp increase in population and modernization of society, environmental pollution resulting from petroleum hydrocarbons has increased, resulting in an urgent need for remediation. Biodegradation of petroleum hydrocarbons in cold environments, including alpine soils, is a result of indigenous coldadapted microorganisms able to degrade. Hydrocarbon oxidizing bacteria have potential applications in bioremediation of oil pollution, enhanced oil recovery, production of surfaceactive agents, and in the use of hydrocarbons as substrates for industrial fermentation processes. Petroleum hydrocarbons can be degraded by microorganisms such as bacteria, fungi, yeast and microalgae 3, 4. Isolation and characterization of crude oil degrading. Master of science in chemistry thesis, department of chemistry, rochester institute of technology, rochester, 108 p. These bacteria were mainly oleibacter, thalassolituus, and alcanivorax genera, all of which include species known to consume aliphatic hydrocarbons. An unexpected enrichment for hydrocarbondegrading bacteria, along with an increase in the abundance and transcription of hydrocarbondegrading genes, was found at depths near the trench bottom 10,400m and 10,500 m.
Ibadan, the results of botany and batch tests have funding available literatures on gum arabic structure on processing different kinds of helene lepaumier and particulate organic. Pdf proliferation of hydrocarbondegrading microbes at. Hydrocarbons are degraded primarily by bacteria and fungi. An overview on microbial degradation of petroleum hydrocarbon. Hydrocarbons persist in antarctic soils when fuel oils such as jp8 jet fuel are spilled. A previously characterized hydrocarbon degrader pseudomonas putida ou83 was used as a positive. Anaerobic degradation of nonmethane alkanes by mbio. Pdf study of oil degrading bacteria isolated from oil. Identification of hydrocarbondegrading bacteria in soil by reverse sample genome probing. Supplementary tables 1,2, figures 16 and references. Since this system was described, alkb homologous have been found in many alkanedegrading. Mar 14, 2018 non hydrocarbon degrading bacteria are isolated from soil sample and subcultured in nutrient broth. These technologies are carried out at a low cost and are more efficient than the physical methods.
Green,1,3 gina freyer,1,2 andy canion, jonathan delgardio, 1nikita. A small number of taxa have been identified as obligate hcb, notably the gammaproteobacterial genera alcanivorax, cycloclasticus, marinobacter, neptumonas, oleiphilus, oleispira, and thalassolituus, as well as the alphaproteobacterial genus thalassospira. Petroleum hydrocarbondegrading bacteria are ubiquitous in nature and can utilize these compounds as sources of carbon and energy. Source of isolation the source for bacterial isolation consisted of wastewaters contaminated with used motor oil, diesel and lubricating oils. Frontiers petroleum hydrocarbondegrading bacteria for the. Effects of temperature and crude oil composition on petroleum biodegradation. Isolation of hydrocarbon degrading bacteria following the strategy designed in our work to isolate hydrocarbon degrading bacteria both with high growth and biological activity, 39 purified bacterial isolates were recorded amongst hundreds of isolated ones at the first step of the program. Enrichment, isolation and identification of hydrocarbon degrading bacteria. Microbial degradation of hydrocarbons in the environment.
Isolation and characterization of hydrocarbon degrading bacterial. The extracellular accumulation of metabolic products by hydrocarbondegrading microorganisms. Isolation, screening and activity of hydrocarbondegrading bacteria from harsh soils. Indeed, the proportion of hydrocarbondegrading bacteria at 10,400 m is the highest observed in any natural environment on earth. Hydrocarbon degrading sulfatereducing bacteria seem to be most versatile in terms of the substrates utilized. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. Floodgate 12 listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi, which were mukherjee an overview on microbial degradation of petroleum hydrocarbon contaminants jahangeer and vikram kumar. These compounds can be transformed by hydrocarbondegrading microorganisms which occur naturally in the environment 3,4. They are known to biodegrade alkanes, alkenes, mono. The purpose of this research was to identify hydrocarbon degrading bacteria which grown on phenanthrene, naphthalene, trichlorodibenzofuran and benzoapyrene. Isolation, identification and characterization of oil. These isolates were purified from soil samples on the basis of their colony morphology, texture, growth, temperature and hydrocarbon utilization ability.
The aim of this study is to identify hydrocarbondegrading bacteria in petroleumcontaminated soil at suame magazine. Awspt 1042 history, soil properties and weather conditions, affecting the occurrence, stability and biological activities of hydrocarbon degrading bacteria 5, 6. Realtime pcr is now widely employed for measuring 16s rrna and functional gene abundance and expression in the environment, has been used in numerous studies of hydrocarbon degrading bacteria, and the technique has promising possibilities as a tool for assessing hydrocarbon contaminated environments and monitoring natural attenuation or. Results the result of the bacterial count showed 38.
Our primers amplified cd gene sequences in naturally occurring hydrocarbon degrading bacteria isolated from groundwater contaminated with gasoline. For instance, some bacteria can metabolize specific alkanes, while others break down aromatic or resin fractions of hydrocarbons. Isolation and molecular identification of hydrocarbon degrading bacteria from oilcontaminated soil fatiha dilmi 1, 2, abdelwaheb chibani 1, khadidja senouci rezkallah 2 1laboratory of microbiology and plant science, department of biology, faculty of natural and life sciences, university of abdelhamidibn badis, mostaganem, algeria. Crude oil and petroleum is a complex mixture of hydrocarbons, which cause widespread environmental contaminants following a spill. Lutibacterium is a genus of gramnegative staining bacteria. For cleanup of hydrocarboncontaminated soils in antarctica, bioremediation has been proposed using hydrocarbondegrading microbes indigenous to antarctic. Isolation, identification and characterization of oil degrading bacteria isolated 431 biochemical characterization of selected microorganisms. The hydrocarbon degrading bacterial types and numbers were found to be lesser than their heterotrophic counterparts. Reconstructing metabolic pathways of hydrocarbondegrading bacteria from the deepwater horizon. Characterization of hydrocarbondegrading bacteria isolated from oilcontaminated sediments in the sultanate of oman and evaluation of bioaugmentation and biostimulation approaches in microcosm experiments. For a long time, the mechanism considered to be prevalent for oil degradation in petroleum reservoirs was the well documented aerobic microbial metabolism and it has long been thought that the flow of oxygen through meteoric waters was necessary for inreservoir petroleum biodegradation. Several bacteria are even known to feed exclusively on hydrocarbons 11. Screening and isolation of a hydrocarbon degrading bacteria by enrichment culture technique.
Hydrocarbon degrading bacteria medina agriculture products. The goal of this project was to evaluate the effects of test conditions and bacterial growth conditions on the results and precision of the math test using a single species of hydrocarbon degrading bacteria. Similarly, in preliminary study 57 oil degrading bacteria were isolated from oil impregnated soil in search of potent hydrocarbon degrader 14. Isolation and identification of effective hydrocarbon. Isolation and molecular identification of hydrocarbon. Pdf the present study was conducted to determine the isolation, identification and characterization of efficient oil degrading bacterial strains. Reconstructing metabolic pathways of hydrocarbondegrading. Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbondegrading activities of microbial communities, in particular by a recently discovered group of specialists, the hydrocarbonoclastic bacteria hcb. Bushnell and hass broth was used as selective enrichment medium with engine oil as a sole carbon and energy source and 19 different isolates were obtained. N, isolation and identification of petroleum hydrocarbon degrading bacteria from oil contaminated soil samples the main factors that affect the effect of flasks and add one gram of hydrocarbon such as remediation include the ph value, soil moisture, benzene, toluene and xylene. The 16s rrna gene sequence of strain pp31t showed 98. Adaptation by prior exposure of microbial communities to hydrocarbons increases hydrocarbon degradation rates.
Hydrocarbon degrading microorganisms were isolated from ten oil polluted areas in and around navi mumbai. Pdf with the sharp increase in population and modernization of society, environmental pollution resulting from petroleum hydrocarbons has. Pollution at the magazine is at a high peak making this place suitable for this research. The hydrocarbo n degrading bacterial counts for the.
Effect of high pressure on hydrocarbondegrading bacteria. Interest in possible hydrocarbon oxidation by sulfatereducing bacteria arose from their frequent presence in oil production. S1 supporting information succession of hydrocarbondegrading bacteria in the aftermath of the deepwater horizon oil spill in the gulf of mexico eric a. Sample was taken from beach simulator tank in marine biotechnology, kamaishi, iwateken, japan on november 2005. Isolation and characterization of hydrocarbon degrading. Alcanivorax borkumensis was the first hcb to have its genome sequenced. In fact, this represents the main origin of failure of most of the bioremediation applications for cleaning polluted areas with hydrocarbons. This phenomenon is related to the chemical structure of petroleum hydrocarbon components. Bacteria displaying such capabilities are often exploited for the.
Degradation of mixtures of aromatic and chloroaliphatic. Created using powtoon free sign up at create animated videos and animated presentations for free. Pdf background the mariana trench is the deepest known site in the earths oceans, reaching a depth of 11,000 m at the challenger deep. The ability of microbial consortia to methanogenically degrade hydrocarbons has already been described, but so far, it has been thought to rely on the syntrophic interaction of hydrocarbon degrading bacteria with methanogenic archaea 24, 25, 57. Isolation of hydrocarbon degrading bacteria from soils.
It may cause extensive damage to the plant physiology and human health. Sample collection sediment samples were collected from the munakkal beach trichur dist, kerala located on the west coast of india immediately after the major event tsunami in 2004. One potent gram negative bacillus bacteria was isolated on the basis of petroleum hydrocarbon degradation efficiency and identified as pseudomonas sp. Pdf petroleum hydrocarbondegrading bacteria for the. Petroleum hydrocarbondegrading bacteria for the remediation of. In the present study, altogether 32 morphologically distinct bacteria were isolated from crude oil contaminated soil and 18 different hydrocarbondegrading bacteria were number. Altogen labs has developed active bioremediation technology based on natural oildegrading bacteria for largescale remediation of hydrocarboncontaminated water and soil. Characterization of hydrocarbondegrading microbial populations. Pdf aromatic hydrocarbondegrading bacteria from soil. Evaluating the detection of hydrocarbondegrading bacteria. Pdf hydrocarbon degrading microorganisms were isolated from ten oil polluted areas in and around navi mumbai. Also, samples amended with cp were found to have more types and higher numbers of heterotrophic and hydrocarbon degrading bacteria than the samples without amendment controls. To 1ml of the bacterial supernatant, 1ml of 20% tca was added and kept for half hour incubation. It includes the hydrocarbondegrading strain lutibacterium anuloederans lc8 references.
Proliferation of hydrocarbondegrading microbes at the. Mittal and singh 2009, have reported eleven petroleum hydrocarbon degrading bacterial isolates from oil production site of lingala oil field, ongc and nine isolates from oil contaminated soil of local area at haridwar. Nonhydrocarbon degrading bacteria are isolated from soil sample and subcultured in nutrient broth. A total of 1 strains of hydrocarbon degrading bacteria were isolated from the surface sediment samples, during the period between july 2009 june 2010, slightly predominated by gramnegative bacteria. Isolation and identification of effective hydrocarbon degrading bacteria from diesel polluted soil doi. Isolation, screening and activity of hydrocarbondegrading. Isolation, screening and activity of hydrocarbondegrading bacteria. Realtime pcr approaches for analysis of hydrocarbon. Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbon degrading activities of microbial communities, in particular by a recently discovered group of specialists, the hydrocarbonoclastic bacteria hcb. Pdf extraction and characterization of oil degrading bacteria. Evaluating the detection of hydrocarbondegrading bacteria in.
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